The aim of this work is the development of a microdevice able to provide in-vitro assays at single cell level. Two modules, integrated in a single platform, are presented: interdigitated electrode arrays (IDEs)-based microsystem for the cell addressed delivery of bio-functionalized nano/microparticles and a cell size microelectrode array (MEA) for single cell electroporation. Both the modules are characterized by two levels of metal structures (buried connection lines made of Al 1% Si + Ti/TiN and gold electrodes) in order to reduce the fabrication costs and the dimensions while improving the device electrical performances. Additional steps of bulk micromachining are developed in order to realize the inlet microfluidics of the MEA-based module. Biocompatible polymers and quartz are used for microchannels and cells confinement respectively. In order to demonstrate the feasibility of this approach, both modules are individually characterized. The dielectrophoretic (DEP) capability of the former is demonstrated by using polystyrene microbeads and the bioaffinity of the latter is evaluated by successful Chinese Hamster Ovary (CHO) cells culture on chip. Moreover, preliminary results of electrochemical impedance spectroscopy [100Hz–1MHz] and of a Randles-based electrical model show the stability of electrode/solution interface parameters (│Z(f)│dispersion < 3%) before and after the cell culture.
Fabrication and characterization of a fully integrated microdevice for in-vitro single cell assays
Collini, Cristian;Morganti, Elisa;Cunaccia, Romina;Odorizzi, Lara;Ress, Cristina;Lorenzelli, Leandro;De Toni, Alessandro;
2009-01-01
Abstract
The aim of this work is the development of a microdevice able to provide in-vitro assays at single cell level. Two modules, integrated in a single platform, are presented: interdigitated electrode arrays (IDEs)-based microsystem for the cell addressed delivery of bio-functionalized nano/microparticles and a cell size microelectrode array (MEA) for single cell electroporation. Both the modules are characterized by two levels of metal structures (buried connection lines made of Al 1% Si + Ti/TiN and gold electrodes) in order to reduce the fabrication costs and the dimensions while improving the device electrical performances. Additional steps of bulk micromachining are developed in order to realize the inlet microfluidics of the MEA-based module. Biocompatible polymers and quartz are used for microchannels and cells confinement respectively. In order to demonstrate the feasibility of this approach, both modules are individually characterized. The dielectrophoretic (DEP) capability of the former is demonstrated by using polystyrene microbeads and the bioaffinity of the latter is evaluated by successful Chinese Hamster Ovary (CHO) cells culture on chip. Moreover, preliminary results of electrochemical impedance spectroscopy [100Hz–1MHz] and of a Randles-based electrical model show the stability of electrode/solution interface parameters (│Z(f)│dispersion < 3%) before and after the cell culture.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
