Monitoring the bioelectrochemical activity of living cells with sensor array-based microsystems represents an emerging technique in a large area of biomedical applications, ranging from basic research to various fields of pharmacological analyses. The main appeal consists in the availability of miniaturised microsystems to perform, in real time, non-invasive in vitro investigations of the physiological state of a cell population. In this paper, we present two different microsystems designed and realised for multisite monitoring of the physiological state of a cell population. The first, intended for cellular metabolism monitoring, consists of an array of 12 spatially distributed ISFETs to detect small pH variations, induced by the cell population, into a defined micro-volume. The second one consists of an array of 40 ISFETs and 20 gold microelectrodes and it has been designed to monitor the electrical activity of neurons. This is obtained by a direct coupling of the neuronal culture with the ISFET sensitive layer and by utilising the gold microelectrodes for the electrical stimulation of the neurons.
Development of ISFET Array-Based Microsystems for Bioelectrochemical Measurements of Cell Populations
Lorenzelli, Leandro;Margesin, Benno;Zen, Mario
2001-01-01
Abstract
Monitoring the bioelectrochemical activity of living cells with sensor array-based microsystems represents an emerging technique in a large area of biomedical applications, ranging from basic research to various fields of pharmacological analyses. The main appeal consists in the availability of miniaturised microsystems to perform, in real time, non-invasive in vitro investigations of the physiological state of a cell population. In this paper, we present two different microsystems designed and realised for multisite monitoring of the physiological state of a cell population. The first, intended for cellular metabolism monitoring, consists of an array of 12 spatially distributed ISFETs to detect small pH variations, induced by the cell population, into a defined micro-volume. The second one consists of an array of 40 ISFETs and 20 gold microelectrodes and it has been designed to monitor the electrical activity of neurons. This is obtained by a direct coupling of the neuronal culture with the ISFET sensitive layer and by utilising the gold microelectrodes for the electrical stimulation of the neurons.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
