A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed inEscherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2, Cd2, Pb2, Cu2 and Zn2 ions in concentration range of 100 fM/10 mM, and the order of sensitivity was SZn/SCu/SHg/SCd/SPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.
Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection
Mortari, Alessia;
2002-01-01
Abstract
A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed inEscherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2, Cd2, Pb2, Cu2 and Zn2 ions in concentration range of 100 fM/10 mM, and the order of sensitivity was SZn/SCu/SHg/SCd/SPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.File in questo prodotto:
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