Mid-infrared ATR FT-IR microspectroscopy is applied in conjunction with multivariate statistical analysis on petal samples of Petunia hybrida from wild-type and from two transgenic lines in which the PhEXPA1 expansin gene expression was down-regulated and up-regulated, respectively. New insights are given on the role of expansin in the rearrangement of the cell wall polymer network. Measurements are done without any previous sample treatment to preserve the native cell wall structure and natural biological variability. An original combination of data analysis techniques is proposed to highlight molecular processes in plant cells, based on an automated spectra selection procedure, Principal Component Analysis, Wilcoxon rank sum test and heat map data representation.

A multivariate statistical analysis approach to highlight molecular processes in plant cell walls through ATR FT-IR microspectroscopy: the role of the alpha-expansin PhEXPA1 in Petunia hybrida

Dell'Anna, Rossana;
2013-01-01

Abstract

Mid-infrared ATR FT-IR microspectroscopy is applied in conjunction with multivariate statistical analysis on petal samples of Petunia hybrida from wild-type and from two transgenic lines in which the PhEXPA1 expansin gene expression was down-regulated and up-regulated, respectively. New insights are given on the role of expansin in the rearrangement of the cell wall polymer network. Measurements are done without any previous sample treatment to preserve the native cell wall structure and natural biological variability. An original combination of data analysis techniques is proposed to highlight molecular processes in plant cells, based on an automated spectra selection procedure, Principal Component Analysis, Wilcoxon rank sum test and heat map data representation.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11582/126601
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
social impact