A novel and unconventional approach to impedance microbiology for the detection of Escherichia coli is under investigation. The detection principle of solution conductivity variation is based on intracellular content escape resulting from bacteriophage generated cell lysis. Bare electrodes on chip included in a PDMS chamber were applied to the impedance spectra measurements. Proofs of principle experiments were performed. In parallel, paramagnetic nano-beads were functionalised with selective phages for sample magnetic concentration and future methods integration. The system potential detection limit is about 10 CFU/chamber and provides the means for selective detection of viable cells only. The methods integration could provide cost–effective results in less than 1 hour.

Proof of principle of a novel impedance microbiology method base on bacteriophages functionalised paramagnetic nanobeads

Mortari, Alessia;Adami, Andrea;Collini, Cristian;Lorenzelli, Leandro
2014

Abstract

A novel and unconventional approach to impedance microbiology for the detection of Escherichia coli is under investigation. The detection principle of solution conductivity variation is based on intracellular content escape resulting from bacteriophage generated cell lysis. Bare electrodes on chip included in a PDMS chamber were applied to the impedance spectra measurements. Proofs of principle experiments were performed. In parallel, paramagnetic nano-beads were functionalised with selective phages for sample magnetic concentration and future methods integration. The system potential detection limit is about 10 CFU/chamber and provides the means for selective detection of viable cells only. The methods integration could provide cost–effective results in less than 1 hour.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11582/248419
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
social impact