This work deals with the development of a disposable miniaturized Polymerase Chain Reaction (PCR) module that will be integrated in an innovative Lab on a Chip (LOC) as Point of Care Testing (POCT) platform to detect the susceptibility of complex diseases with genetic profiling. The amplification system consists of a micro-chamber reactor with a hybrid silicon-polymer structure. The temperature control system has been implemented by means of Platinum microheaters and thermometers integrated on a Silicon substrate and the reaction chamber has been completely made of Polydimethylsiloxane (PDMS) since it is biocompatible, transparent and easily moldable. To assure a perfect sealing, an oxygen plasma bonding process step has been implemented. The device design has been supported by analytical and finite elements simulations in such a way to evaluate the thermal requirements. A first PCR chamber prototype has been fabricated and packaged.
Design and Fabrication of a Micro PCR Module for POC Applications
Morganti, Elisa;Collini, Cristian;Ress, Cristina;Adami, Andrea;Lorenzelli, Leandro
2009-01-01
Abstract
This work deals with the development of a disposable miniaturized Polymerase Chain Reaction (PCR) module that will be integrated in an innovative Lab on a Chip (LOC) as Point of Care Testing (POCT) platform to detect the susceptibility of complex diseases with genetic profiling. The amplification system consists of a micro-chamber reactor with a hybrid silicon-polymer structure. The temperature control system has been implemented by means of Platinum microheaters and thermometers integrated on a Silicon substrate and the reaction chamber has been completely made of Polydimethylsiloxane (PDMS) since it is biocompatible, transparent and easily moldable. To assure a perfect sealing, an oxygen plasma bonding process step has been implemented. The device design has been supported by analytical and finite elements simulations in such a way to evaluate the thermal requirements. A first PCR chamber prototype has been fabricated and packaged.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
