Three different methods of using protein-based capacitive biosensors for the detection of heavy-metal ions are presented. The metal-binding proteins SmtA, S100A12, MerP and four modified MerPs were immobilised as the bio-recognition element on self-assembled monolayer-modified gold electrodes. Capacitance was measured using potential square step or electrical impedance spectroscopy. The protein-metal interaction generated changes in capacitance mainly due to a protein conformational change.
Applications of protein-based capacitive biosensors for the Detection of Heavy-Metal Ions
Mortari, Alessia;
2006-01-01
Abstract
Three different methods of using protein-based capacitive biosensors for the detection of heavy-metal ions are presented. The metal-binding proteins SmtA, S100A12, MerP and four modified MerPs were immobilised as the bio-recognition element on self-assembled monolayer-modified gold electrodes. Capacitance was measured using potential square step or electrical impedance spectroscopy. The protein-metal interaction generated changes in capacitance mainly due to a protein conformational change.File in questo prodotto:
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